Restriction endonuclease Dde Ⅰ traits

Restriction endonuclease Dde Ⅰ is a microbial brand name of the aboriginal letter and the breed name, the aboriginal two belletrist fourth belletrist announce strains (strains). For example, the brake agitator extracted from Bacillus amylolique faciens H is alleged Bam H, admit altered nucleotide arrangement acquired in the aforementioned strains of bacilli of several altered specific enzymes, may be programmed to a altered number, as HindII , HindIII, HpaI, HpaII, MboI, MboI. Brake agitator (restriction endonuclease): aural a specific nucleotide arrangement at the hydrolysis of bifold abandoned DNA endonuclease. The blazon Ⅰ brake endonucleases both catalytic host DNA methylation, the catalytic non-methylation of DNA hydrolysis; blazon II brake enzymes activate the hydrolysis of the non-methylated DNA. Brake endonuclease agitator named; about based on microorganisms case of the aboriginal letter of the name and the aboriginal two belletrist of the breed name fourth belletrist announce strains (strains). For example, the brake agitator extracted from Bacillus amylolique faciens H is alleged Bam H, admit altered nucleotide arrangement acquired in the aforementioned strains of bacilli of several altered specific enzymes, may be programmed to a altered number, as HindII , HindIII, HpaI, HpaII, MboI, MboI.

Restriction endonuclease Dde Ⅰ important acid accoutrement acclimated in abiogenetic engineering. Scientists abandoned from prokaryotes, abounding kinds of brake enzymes, and accept been commercialized and broadly acclimated in abiogenetic engineering. Brake agitator acid characteristics, they can be disconnected into two categories: one is acid locations of the non-specific; added is accurately articular nucleotide arrangement alone in assertive DNA sequences cutting. This can be accurately accustomed by the acid locations accepting a palindromic sequence, that is, in the acid portion, with addition alternation of the nucleotide arrangement of a alternation of advanced apprehend in the adjustment about-face apprehend exactly. The abiogenetic engineering majority afterwards a chic of enzymes. Brake agitator specific acid website for cutting, in accordance with the address of cutting, and can be disconnected into break microburst cut two kinds of peace. The break cut is about cut in two altered locations of the chain, afar by a few nucleotides in the average and the cut ends of the end of the single-chain anatomy a palindrome formula, this end with the purpose of accepting a commutual nucleotide gene DNA fragment link, it is alleged a adhesive end. This agitator applications in abiogenetic engineering.

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