The Tachypiens Amebocyte Lysate
is a antiseptic freeze-drying artefact fabricated of claret amoebocyte lysate
of abyssal organisms, absolute proclotting agitator and coagulogen which are
activated by microbial endotoxin and fungal glucan. The Tachypiens Amebocyte
Lysate is a biological reagent acquired by extracting amoebocyte lysate from
the dejected claret of the limulus which is inhabited by the sea, and
freeze-drying at a low temperature. It accurately and bound determines whether
a sample contains bacterial endotoxin and (1,3)-β-glucan in a qualitative or
quantitative manner.
The main component of
Tachypiens Amebocyte Lysate is the ambit backtalk hemocytes, which is advised
with chloroform to abolish the anti-lipopolysaccharide factor, and the adapted
bulk of divalent calcium and magnesium ions are added, and the C, B, and G
factors are contained.
At present, Tachypiens
Amebocyte Lysate is broadly used in the fields of pharmaceutical, analytic and
accurate analysis for the apprehension of bacterial endotoxin and fungal
glucan. The Amebocyte Lysates currently used are classified into Limulus
Amebocyte Lysate and Tachypleus Amebocyte Lysate.
Tachypleus amebocyte
lysate(TAL)or limulus amebocyte lysate(LAL)has been abundantly used for
bacterial endotoxin test.